Monday, September 9, 2019

Bacteriology Case Study Example | Topics and Well Written Essays - 500 words

Bacteriology - Case Study Example difficile microorganisms. The selected methods must be affordable for the organisation undertaking the testing. Since the different methods require different testing durations, the number of samples and the time results expected also determine the testing methods to be utilised (DuPont 2011). To ensure that all these factors have been met, optional methods can be utilised either singly or by combining two methods. The best option for testing would be combining Glutamate dehydrogenase (GDH) assay with enzyme immune-assay (EIA). The combination of these methods would enhance the limitations experienced by each method and increase the reliability of results. Samples would be prepared and each sample tested using both methods, and results recorded separately. A comparison of the results would then be undertaken to establish the various attributes being analysed by the results. The GDH method has been identified as having high sensitivity, but very poor specificity. The method can, therefore, accurately rule out the presence of clostridium difficile, but cannot ascertain the presence of the microbes (Goldenberg et al. 2010). The method rarely produces a negative for samples labelled true-positive using the EIA testing method. In most testing processes, GDH produces 100% specificity result because of the lack of false positive result, from the testing. The combination of these methods would enhance findings since GDH produces high sensitivity, and EIA produces relatively high specificity. Combining these methods, therefore, presents researchers with a capacity to have reliable results from the laboratory testing of clostridium difficile. GDH accurately tests the presence of enzymes produced by these microorganisms, but cannot ascertain the presence of C. difficile since similar enzymes are produced by other bacteria (Eastwood et al. 2009). The method can, therefore, rule out the presence of the microbial

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